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Sea Urchin
Gamete collection
Spawning
can be induced in adult sea
urchinsby injecting 1 ml of
a0.5M KCl solution into
several sites in the soft
membrane around the mouth. Within minutes, the gametes
should appear: the sperm are
off-white, the eggs are tan to orange. Collect the sperm by
inverting the male onto a
small, dry petri dish. After several minutes, cover the
sperm and put on ice. Collect
the eggs by inverting the female onto a small beaker filled
with ASW
(room temperature for L.
variegatus, and below 14ºC for S.purpuratus and
L.pictus ). Keep urchins from falling in
with aluminum foil supports.
Allow eggs to settle, pour off ASW and replace with fresh
ASW (at same temperature).
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