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Solutions for Sea Urchin

ASW: Artificial sea water (MBL)

NaCl

24.72 g

KCl

0.67 g

CaCl2.2H2O

1.36 g

MgCl2.6H2O

4.66 g

MgSO4.7H2O

6.29 g

NaHCO3

0.18 g

dH2O

to 1 liter


Sulfate-free Sea water

NaCl

27.72 g

KCl

0.67 g

CaCl2.2H2O

1.36 g

MgCl2

9.32 g

NaHCO3

0.18 g

dH2O

to 1 liter


Development

0.5 M KCl to induce gamete shedding

3.73 g KCl/ 100 ml dH2O

LSSW: Low sodium sea water (50 mM)

NaHCO3

0.2 g

Choline Cl

28.3 g

KCl

0.7 5g

CaCl2.2H2O

1.6 g

MgCl2.6H2O

5.5 g

MgSO4.7H2O

7.1 g

dH2O

to 1 liter, adjust pH to 7.8

CaFSW: Calcium-free Seawater

NaCl

25.6 g

KCl

0.7 g

MgSO4.7H2O

11.9 g

NaHCO3

0.5 g

dH2O

to 1 liter


© 2000 Cebra-Thomas

Last Modified: 25 May, 2004


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