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5. Once the surgery is complete, be sure to take pictures of a normal donor and recipient and then the newly transplanted graft of the recipient. Pictures should be taken at various stages to observe development (to be determined at discretion of researcher).
6. The embryos should be left in HBSt at room temperature to continue to development. Observe both donors and recipients as time continues to examine the effect of the transplanted graft.
7. Make OBSERVATIONS as development continues...this is the key to obtaining results!
8. After the first day, it is important to replace the operating solution with 10% modified Steinberg's solution. The graft will develop better in a lower concentration of certain chemicals. To do this, simply pipette off the operating solution and pour in the 10% modified Steinberg's solution.

© Cebra-Thomas 2001

Last Modified: 5 August 2001


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