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Fertilization The eggs were washed several times with ASW by allowing them to settle and pouring off the water. This will removed some of the jelly coat. Enough eggs to cover the bottom of a 50 ml beaker were transfered along with 10 ml of 10mM p-aminobenzoic acid in ASW. 50 ml of sperm were diluted in 5 ml of ASW. 2 drops were used to fertilize eggs. The presence of the fertilization envelope was checked after 5 and 10 minutes in the control culture. The eggs were poured through a nylon filter mounted to the end of a 50 ml beaker. This was repeated until most of the fertilization envelopes have been removed. The cells were allowed to settle for a few minutes.