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Separation of sea urchin blastomeres

Tony Sigismondo, F&M College

to separate the Blastomere at different points in development and observe the growth of the resulting embryos.

Procedure: We extractrf the gametes from the sea urchins, fertilized the sea urchin eggs, and removed the fertilization envelope. The cells were then be allowed to cleave, at which point they were be introduced into a hypertonic seawater solution. This caused the cells to shrink and break apart. Once the majority of the cells had separated, the water was replaced with normal seawater. Their growth was be compared to that of the control group that was not divided.

© Cebra-Thomas, 2000
Last Modified: 24 April 2000

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