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		PROTOCOL 1. Incubate chicken eggs for 2 days. 2. Remove eggs from incubator. Aseptically window eggs(do not remove membrane) and inject control and experimental eggs with 1uL of solution into yolk and away from embryo. a. Control egg solution: 1mL of Howard Ringer's solution, 100uL DMSO (5 mM) b. Experimental egg solution: 1mL of Howard Ringer's Solution, 100 uL SU5402* (5 mM). *handle SU5402 with extreme caution! 3. After windowing and injecting the control and experimental solutions, place in 37ºC incubator for 1 or 2 day(s). 4. Open eggs and remove embryo aseptically. Clean embryos in Howard Ringer's Solution and capture images of experimental and control embryos. 5. Preserve in 4% Paraformaldehyde. 6. Process embros with DIG-labled shhriboprobe formin situhybridization. 7. Capture images ofin situhybridized experimental and control embryos.