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4. Transfer the neural fold transplant on the tip of the glass needle to the ventral pocket on the recipient embryo. Gently and firmly push the whole mass through the narrow incision and anchor it against the yolk. A tight fitting transplant is desirable.

5. Quickly cover the implant with the flat surface of a piece from a thin-grade coverslip. Wait one hour.

6. Carefully remove the coverslip. If the transplant has not healed properly, place it under pressure again for another half hour. After healing is complete, transfer both the donor and the host embryos to a glass dish containing spring water.

7. Observe the differentiation of the implanted tissue. Note whether graft was successfully transplanted, how the embryo responded to the graft, and whether there have been any differences which have occurred in the development of the embryo.

© Cebra-Thomas 2000

Last Modified: 5 August 2001


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