M.S. degree in Environmental Science, University of Maryland College Park, 2004
Developed Random Amplified Polymorphic DNA (RAPD) markers and a high-performance liquid chromatography (HPLC) protocol to quantify genetic and chemical variation in wild and cultivated populations of the rare, medicinal herb, American ginseng (P. quinquefolius).
B.A. degree with honors in Biology, Magna Cum Laude, Colgate University, 1998
MS Research Abstract
American ginseng (Panax quinquefolius) is a valuable medicinal herb threatened predominantly by over-harvest. Because data are insufficient, current attempts to protect diversity and improve cultivation are inadequate. Random Amplified Polymorphic DNA (RAPD) markers were used to estimate genetic diversity and high-performance liquid chromatography (HPLC) was used to characterize root ginsenoside concentrations in wild and cultivated populations of American ginseng in Maryland. Wild populations were less diverse than cultivated and highly differentiated from one another; suggesting that drift was high and gene flow low in wild populations. Plants grown from local seed sources were genetically and phytochemically distinct from plants grown from commercial Tennessee and Wisconsin seed sources. Plants grown from local seed sources exhibited high levels of Rg1 root ginsenoside and low levels of Re root ginsenoside; a ginsenoside profile not previously reported for American ginseng roots. Plants from at least one wild population were genetically and phytochemically similar to exotic commercial plants, suggesting that exotic commercial seed has been introduced into some wild populations. Thus, native Maryland American ginseng is unique but threatened by drift, isolation and artificial introductions.
Schlag, E.M. and M.S. McIntosh. 2012. RAPD-based assessment of genetic relationships among and within American ginseng (Panax quinquefolius L.) populations and their implications for a future conservation strategy. Genetic Resources and Crop Evolution 59(7): 1553-1568.
Schlag, E.M. and M.S. McIntosh. 2006. Ginsenoside content and variation among and within American ginseng (Panax quinquefolius L.) populations. Phytochemistry 67(14): 1510-1519.
Pile, L.A., E.M. Schlag and D.A. Wassarman. 2002. The SIN3/RPD3 deacetylase complex is essential for G2 cell cycle progression and stability of the SMRTER corepressor. Mol. and Cellular Biology 22:4965-4976.
Wassarman, D.A., Aoyagi, N., Pile, L.A. and E.M. Schlag. 2000. TAF250 is required for multiple developmental events in Drosophila. Proc. Natl. Acad. Sci. 97:1154-1159.
Schlag, E.M. and D.A. Wassarman. 1999. Identifying mutations in Drosophila genes by direct sequencing of PCR products. Biotechniques 27:262-263.
Hoham, R.W., E.M. Schlag, J.Y. Yang, A.J. Hasselwander, A.F. Behrstock, R.C. Johnson and S.C. Roemer. 1998. The effects of irradiance levels and spectral composition on mating strategies in the snow alga, Chloromonas sp.-D, from the Tughill Plateau, New York State. Hydrological Processes 12:1627-1639.
Presented a talk titled "Genetic Diversity of Maryland-Grown American Ginseng (Panax quinquefolius L.)" at the Society for Conservation Biology annual meeting, July 2004.