1 2 3 4


...

Results and Discussion

The two samples behaved quite differently. The Ig8 staining revealed that PMCs of the control group had in fact migrated after gastrulation to form the endoskeleton, or spicules, of the urchin. In the experimental group, none of the embryos had undergone complete gastrulation. The Ig8 staining showed that the PMC cells had failed to migrate out, the embryo itself had failed to develop.
Our original hope, that we could block skeleton formation in the urchin, was satisfied by this experiment. We also observed that NiCl
2disrupts not only skeleton formation, but the process of gastrulation as well.



© 2000 Cebra-Thomas

Last Modified: 19 June, 2000


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