1 2 3 4

 

In the second part of the experiment the potential effects of ethanol were examined by keeping a constant amount of ethanol in each sample. Using the data obtained from the first part of the experiment, we knew that at cyclopamine treatments above 100 micromoles there were no surviving embryos making these concentrations useless.

The following concentrations (in micromoles) were used, resulting in a certain number of mutations, deaths, and normal growths.

spacer

Control

50 cyclopamine

50 ethanol control

100 cyclopamine

100 ethanol control

Normal

15

6 w/ minor changes

7 w/minor changes

0

0

Mutated

0

3 major

3 major

8

0

Dead

0

6

5

7

15

©Cebra-Thomas, 2000

Last Modified: 12 May 2000

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