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First Experiment
After treatment with 1.0 mg of nicotine, all 24 chick embryos were dead. The embryos were removed, but no development could be determined. The 6 control embryos were still alive.

Second Experiment
After approximately 24 hours following treatment, 2 of the embryos from the 0.5 mg treatment group were dead. By 48 hours 6 more embryos from the0.5 mg group were dead and 9 embryos from the 0.25 mg group were dead. At 72 hours after treatment, two more embryos, one from each group, had died. When examined, the two embryos revealed developmental retardation in the cranial region, limbs, and eyes
(Image 1). 3 days following the second treatment 3 more embryos from the 0.5 mg group were dead. At this point, all embryos from the 0.5 mg group were dead and 2, 0.25 mg nicotine treated embryos remained. On day 6 following the second treatment both 0.25 mg treated embryos had died. All controls were still viable. Treatment continued until day 13 when the 2 remaining embryos were removed and examined (Image 2). To prevent further contamination, the experiment was stopped 5 days short because of the presence of mold in 2 of the controls. One embryo (egg #8) exhibited low body length and birth weight when compared to the control group (Image 3). The second embryo (egg #9) displayed an apparent redness of the skin. Veins appeared to protrude from the surface of the body. (Image 4). All controls exhibited normal development (Image 5).

©Cebra-Thomas, 2000

Last Modified: 5 May 2000

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