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There were three different trials run in order to see if we could get successful neural crest cell migration. All of our control wells showed a large amount of neuron differentiation, with the neurite formation quite visible after staining with anti-neurofilament antibody. Most of the neurons that formed in the control wells were normal in appearance with only one main neurite branch. The two wells containing 1 mM Pb-Acetate showed a few neurons that had differentiated. These neurons seemed to have one main neurite with multiple branches. The two wells with 0.1 mM Pb- Acetate had a considerable number of differentiated neurons. In one well, there were two large webs of neurons, most of which had multiple branches coming off of each neuron axon body (see figures below).

Control neural crest cultures

Neural crest cultures treated with lead acetate

©Cebra-Thomas, 2000

Last Modified: 5 May 2000

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