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expression. The experimental embryos exhibited a greater amount of shh staining in both the limb buds and craniofacial region than the control embryos. In the limb buds the domain of shh expression extended to the anterior portion of the limb bud, whereas in the controls shh was confined o the posterior portion of the limb buds. Both the control and experimental embryos show staining along the notochord (Figure 3).

(a)control/shh (b)SU5402/shh
Figure 3. Embryos processed with DIG
labled shh antisense probe. (a) embryo
treated with DMSO exhibits less shh
expression (b) interfering with FGF
signaling resulted in greater shh
expression in the craniofacial and
limb bud regions(white arrows) as
well as the branchial arches.

. . .

©Cebra-Thomas, 2001

Last Modified: 2 August 2001

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