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and an experimental 5x10-3M solution of the SU5402. One day after injection of solutions the embryos were photographed and fixed in 4% paraformaldehyde. An in situ hybridization was performed on a control and experimental embryo where they were stained for SHH. The experimental embryos were found to have defects craniofacial defects. In situ hybridization of the experimental embryos revealed increased shh expression along the notochord, limb buds, and craniofacial region. This could signify the build up of SHH due to the interference of FGF signaling. The results reinforce the importance of FGF signals in chick development and the ability of SU5402 to interfere with FGFRs.

©Cebra-Thomas, 2001

Last Modified: 2 August 2001

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