Solutions for Chick Embryology 10XTyrode's saline 80 gNaCl 2 gKCl 2.71 gCaCl2.2H2O 0.5 gNa PO4.2H2O 2 gMgCl2.6H2O 10 g glucose dH2O to 1 liter, autoclave 4% PFA in PBS 100 ml 1X PBS (heated to 60ºC, stirring) 4 g paraformaldehyde 0.5 ml 1 N NaOH 0.5 ml 1N HCl (add when PFA dissolved) ©Cebra-Thomas, 2000 Last Modified: 13 April 2000 [Lab Protocols | Students | Cebra-Thomas | Course | Links ]
				Ca+2/Mg+2 free Tyrode's solution 8.0 g NaCl 0.3 gKCl 0.05 gNaH2PO4.H2O dH2O to 1 liter
				Howard Ringer's solution 7.2 g NaCl 0.17g CaCl2(anhyd.) or 0.23g CaCl2.2H2O 0.37g KCl dH2O to 1 liter