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		Mouse Development: Gonad Removal and Germ Cell Staining Stephanie Ferrari Objective:To remove the gonads, determine the sex ofmouse embryos 13.5 days post coitum and visualize the germ cells, through use of an alkaline phosphatase staining. The sex of the embryos can be determined by visually observing the gonads. Alkaline phoshatase staining allows for the visualization of the germ cells.