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Prep list for RNA isolation, RNA gel and cDNA synthesis


10X MOPS buffer:

To 400 ml DEPC-treated dH2O, add 20.9 g MOPS 8.3 ml 3 M NaAcetate
10 ml 0.5 M EDTA, pH 8
pH to 7.0 with 10N NaOH or acetic acid Cover with aluminum foil and autoclave

Howard Ringer's solution
7.2 g NaCl
0.17g CaCl
2(anhyd.) or 0.23g CaCl2.2H2O
0.37g KCl
add dH
2O to 1 liter

5X TBE buffer
54 g Tris base
27.5 g boric acid
20 ml 0.5 M EDTA, pH 8
2O to 1 liter

Polytron homogenizer
Safety goggles
Lab coats
Variable speed microfuge
P1000, P200 and P20 pipetmen
UV spectrophotometer and cuvette
Water bath (41ºC)
Shaking incubator (55ºC and 65ºC)
Heating stirrer
Power supply
RNase-free minigel box

Small, medium & large gloves
Bench paper
Petri dishes (sterile)
Pipetmen tips (barrier, sterile & RNase-free) for P1000, P200 and P20 Microfuge tubes (sterile & RNase-free)
8 ml tubes (sterile)
Polypropylene bags for chemical waste
Storage box
Tube poppers

@2004 Cebra-Thomas

Last Modified: 17 August 2004

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