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Zebrafish Embryo solutions From: The Zebrafish Book, University of Oregon

Embryo Medium

(make fresh weekly)

Stock #11.0 ml Stock #20.1 ml Stock #41.0 ml ddH2O 96.5 ml Stock #51.0 ml NaHCO30.35 g Use about 10 drops of 1 MNaOH to bring to pH 7.2

Hank's Stock #1 NaCl KCl ddH2O to 100 ml Hank's Stock #2 Na2HPO4*7H2O KH2PO4 ddH2O to 100 ml

Hank's stock #4 CaCl2*2H2O ddH2O to 100 ml

8.0 g 0.4 g

1.92 g

Hank's Stock #5 MgSO4*7H2O ddH2O to 100 ml

0.67 g 0.60 g

2.46 g

Note:use ultrapure dH2O throughout