Solutions for Chick Embryology


10X PBS stock

NaCl

175.3g

KH2PO4

7.7g

K2HPO4 (anhydrous)

25 g

dH20

to 2L



10X Tyrode's saline

NaCl

80 g

KCl

2 g

CaCl2.2H2O

2.71 g

Na PO4.2H2O

0.5 g

MgCl2.6H2O

2 g

glucose

10 g

dH2O

to 1 liter, autoclave


4% PFA in PBS

Heat 100 ml 1X PBS to 60ºC

Add 4 g paraformaldehyde with stirring

Add 0.5 ml 1 N NaOH and stir until clear

Neutralize with 0.5 ml 1N HCl

 

©Cebra-Thomas, 2000

Last Modified: 25 May 2005

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Ca+2/Mg+2 free Tyrode's solution

NaCl

8.0 g

KCl

0.3 g

NaH2PO4.H2O

0.05 g

dH2O

to 1 liter


Howard Ringer's solution

NaCl

7.2 g

CaCl2(anhyd.)

0.17g

or CaCl2.2H2O

0.23g

KCl

0.37g

dH2O

to 1 liter